日韩精品人妻一区二区中文,五月婷婷综合在线视频,A久久精品国产精品亚洲,亚州中文精品有码视频在线,精品1区2区3区产品乱码,色哟哟免费观看视频入口,婷婷射精AV这里只有精品,2024日产乱码国产,国产精品三级一区二区,艳妇乳肉豪妇荡乳AV

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pUB110cop1
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pUB110cop1
pUB110cop1
規(guī)格:
貨期:
編號(hào):B236565
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUB110cop1
商品貨號(hào) B236565
Designations pUB110cop1
Depositors H Leonhardt
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pUB110cop1 (plasmid)
Promoters: Promoter none
Construction: pUB110, pEB114cop1
Marker(s):kanR,neoR
Construct size (kb): 4.5
Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110
Applications
shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5.
The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability.
Low copy number shuttle vector.
Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130.
Media ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
芜湖县| 依安县| 阜城县| 武宣县| 布尔津县| 清苑县| 太保市| 灵璧县| 长岭县| 鸡西市| 驻马店市| 文登市| 乐清市| 正安县| 谢通门县| 东宁县| 永和县| 原平市| 盈江县| 胶州市| 平度市| 平顶山市| 榆中县| 镇康县| 隆德县| 永修县| 泸水县| 大方县| 太仆寺旗| 铜鼓县| 凉山| 临澧县| 崇阳县| 神木县| 惠州市| 满洲里市| 宁德市| 洛隆县| 迁西县| 蚌埠市| 潢川县|