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pDK4
pDK4
規(guī)格:
貨期:
編號(hào):B234172
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDK4
商品貨號(hào) B234172
Designations pDK4
GenBank Number

M97755

Species Homo sapiens, human
Depositors SR Grimes
Vector
Construct size (kb): 3.543999910354614
Insert
DNA: genomic
Insert lengths(kb): 0.8579999804496765
Tissue: placenta
Gene product: H1 histone, testicular [H1T*]
Insert Size (kb) 0.858
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Some restriction sites not found in the insert: BamHI BglI BglII EcoRI HaeII HincII KpnI NcoI NotI PvuI PvuII SacI XbaI XhoI.
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.7, 0.9; BglI--2.4, 1.2; HindIII--3.0, 0.5; PstI--2.8, 0.75; SmaI--3.5; PvuI/HindIII--1.6, 0.96, 0.66, 0.43; BglI/HindIII--1.4, 1.1, 0.66, 0.43.
The insert contains the following restriction sites (bp from the 5' end): AvaI--86, 96; HindIII--477; PstI--166; SmaI--96.
The insert is a pcr product amplified from human placental DNA.
The insert is in the opposite orientation as lacZ'.
References

Koppel DA, et al. Primate testicular histone H1t genes are highly conserved and the human H1t gene is located on chromosome 6. J. Cell. Biochem. 54: 219-230, 1994. PubMed: 8175896

Sidney R Grimes, personal communication

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