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Construct size (kb): 4.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: G22P2*
Gene name: thyroid autoantigen 86kD (Ku antigen)
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M77907; EST: EST01491; Primer: M13 Forward;
THC id: THC124004

Insert lengths(kb): 1.200000047683716

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: thyroid autoantigen 86kD (Ku antigen) [G22P2*]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; PstI--4.2; HindIII--4.2.

EST01491 matches GenBank accession HUMKUANT with 99.65% identity over the sequence range: 1-289.