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H57-597
H57-597
規(guī)格:
貨期:
編號(hào):B164542
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H57-597
商品貨號(hào) B164542
Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Derivation
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor
Comments
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells.
The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG
Name of Depositor RT Kubo
Deposited As hamster, Armenian (B cell); mouse (myeloma)
References

Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936

White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300

Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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