日韩精品人妻一区二区中文,五月婷婷综合在线视频,A久久精品国产精品亚洲,亚州中文精品有码视频在线,精品1区2区3区产品乱码,色哟哟免费观看视频入口,婷婷射精AV这里只有精品,2024日产乱码国产,国产精品三级一区二区,艳妇乳肉豪妇荡乳AV

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > C1R-sB7
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
C1R-sB7
C1R-sB7
規(guī)格:
貨期:
編號:B164078
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 C1R-sB7
商品貨號 B164078
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties mixed; semi-attached
Biosafety Level 2 [Cells contain Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation

C1R-sB7 is a stable transfectant cell line established in 1988 with a modified histocompatibility complex (MHC) HLA B7 gene. It secretes HLA B7 but does not express surface HLA B7.

C1R-sB7 was established by transfection by electroporation of the C1R cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo carrying the gene for soluble B7 (sB7). It was selected in geneticin and cloned by limiting dilution. 

C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.

Genes Expressed
Secretes HLA B7 but does not express surface HLA B7
Cellular Products
Secretes HLA B7 but does not express surface HLA B7
Comments
C1R-sB7 secretes HLA B7 but does not express surface HLA B7.

C1R-sB7 has been a reference standard in International Workshops on soluble HLA.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured by scraping.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
Name of Depositor F Grumet
Year of Origin 1988
References

Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569

Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Grumet FC, et al. Report of the second international soluble HLA (sHLA) workshop. Phoenix, Arizona, October 2, 1993. Hum. Immunol. 40: 153-165, 1994. PubMed: 7960956

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
稻城县| 临夏县| 彭山县| 泰宁县| 徐水县| 阳东县| 泰州市| 桐梓县| 海安县| 山丹县| 玉环县| 贡觉县| 招远市| 云和县| 内江市| 龙井市| 固安县| 雷州市| 安康市| 台南县| 衡东县| 高要市| 三台县| 玉屏| 维西| 博乐市| 西华县| 沈阳市| 资阳市| 乐都县| 旺苍县| 黄平县| 呼伦贝尔市| 梓潼县| 罗源县| 壶关县| 武川县| 延川县| 和硕县| 交城县| 福海县|