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pLNbovIL-2 [pLNbovIL2]
pLNbovIL-2 [pLNbovIL2]
規(guī)格:
貨期:
編號(hào):B162208
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pLNbovIL-2 [pLNbovIL2]
商品貨號(hào) B162208
Designations pLNbovIL-2 [pLNbovIL2]
GenBank Number

M12791

Species Bos taurus, cow
Depositors Immunex Corporation, S Gillis, Immunex Corporation
Applications
produces protein interleukin 2
Vector
Construct size (kb): 6.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pPL-lambda
Intact vector size: 5.218
Type of vector: plasmid
Vector end: HpaI
Vector end: HpaI
Cloning sites: HpaI
Polylinker sites:
Construction: pBR322, lambda
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: lambdaPL
replicon: pMB1
terminator: lambda tL1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: bovine
Gene symbol: IL2
Genomic copy number: unique
Gene name: interleukin 2
Contains complete coding sequence?: U
Tissue: lymph node, Con A-stimulated
Type of DNA: cDNA
Insert end: HgiAI
Insert end: PstI
Insert size (kb): 0.75
Cross references: DNA Seq. Acc.: M12791
Insert lengths(kb): 0.75
Tissue: lymph node, Con A-stimulated
Gene product: interleukin 2 [IL2]
Target Gene: interleukin 2
Insert Size (kb) 0.75
Media ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of pLNbovIL-2 give the following sizes (kb): EcoRI--6.2; SalI--6.2; NcoI--6.2; XbaI--6.0, 0.2; XbaI/SphI--4.3, 1.7, 0.2.
Restriction digests of pRK248cIts give the following sizes (kb): EcoRI--11.0; SalI--11.0; NcoI--10.5, 0.5; XbaI--uncut; SphI--11.0.
Contains 2 plasmids: pLNbovIL2 (6.2 kb, ampicillin resistant) and pRK248cIts (ATCC 33766) (11.0 kb, tetracycline resistant), encoding the temperature-sensitive lambda cI repressor used to regulate expression from pLNbovIL2.
pRK248cIts is present at lower copy number and can usually be distinguished from pLNbovIL-2 on agarose gels stained with ethidium bromide.
Detects bovine genomic restriction fragments of the following sizes (kb): BamHI--10.0; EcoRI--5.8, 5.0; HindIII--5.1, 3.3, 1.9.
After thermal induction, this construct produces biologically active product.
The HgiAI/PstI fragment encoding aa 22 to 135 from pIL-2-4 was blunt-ended and inserted into a construct based on pPL-lambda already containing an oligonucleotide providing a ribosome binding site, an initiator Met codon, and a codon for Ala 21.
References

Cerretti DP, et al. Cloning, sequence, and expression of bovine interleukin 2. Proc. Natl. Acad. Sci. USA 83: 3223-3227, 1986. PubMed: 3517854

Anderson DM, et al. DNA sequences encoding bovine interleukin-2. US Patent 4,882,282 dated Nov 21 1989

Douglas P Cerretti, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
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